The difference between PCIe x1, x4, x8, x16 and x32

Yes, a x1 card should work in a x16 slot It's good that you've got it to work in a x1 slot What else have you tried it in besides the x16 slot

x1 speed, while a full-size PCIe slot will run at x16 PCIe x16 slots are generally intended for use with Graphics Cards PCIe 1x slots are generally for

pcie slot height 1 PCIe x16, 1 PCIe x16, 1 PCIe x1, 1 Key E for WiFi - 1 x PCI Express x1 Slot - Supports AMD Quad CrossFireX™ and CrossFireX

pcie 2 slot PCIe x1: This is the smallest PCIe slot and has a single lane for data communication PCIe x1 slots are often used for less demanding expansion cards, such as